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quantikine elisa kit  (R&D Systems)


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    R&D Systems quantikine elisa kit
    Quantikine Elisa Kit, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 68 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/quantikine+kits/Human+M-CSF+Quantikine+ELISA+Kit/pm42821460-65-17-22
    Average 94 stars, based on 68 article reviews
    quantikine elisa kit - by Bioz Stars, 2026-10
    94/100 stars

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    Related Articles

    Biomarker Discovery:

    Article Title: Bifidobacterium longum able to beneficially modulate immune response to respiratory virus infection
    Article Snippet: .. The concentrations of mouse IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-12/IL-23p40, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E, IL-17F, IL-21, IL-22, IL-23, IL-30, IL-31, IL-33, IP-10, MIP3α, MIP-2, MIP-1β, MIP-1α, MCP-1, KC/GRO, TNF-α, VEGF, EPO, GM-CSF, IFN-γ in both serum and BAL fluid were measured using a commercial U-PLEX Biomarker Group 1 Mouse 35-Plex (MesoScale Discovery) platform following the manufacturers' instructions; mouse IL-28 (IFN-k 2/3), mouse G-CSF, mouse TRAIL, mouse AREG were detected using ELISA kits (RayBiotech, Inc,); Oncostatin M and mouse surfactant protein D (SPD) were measured using Quantikine kits from R&D Systems following the manufacturer's instructions. .. Mouse IFN-α was measured in serum and BAL fluid by mouse IFN alpha platinum ELISA (ThermoFisher scientific) Serum and BAL fluid levels of mouse interferon-β (IFN-β) were measured using VeriKine Mouse Interferon Beta HS ELISA Kits (PBL Assay Science).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Bifidobacterium longum able to beneficially modulate immune response to respiratory virus infection
    Article Snippet: .. The concentrations of mouse IL-1β, IL-2, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12p70, IL-12/IL-23p40, IL-13, IL-15, IL-16, IL-17A, IL-17A/F, IL-17C, IL-17E, IL-17F, IL-21, IL-22, IL-23, IL-30, IL-31, IL-33, IP-10, MIP3α, MIP-2, MIP-1β, MIP-1α, MCP-1, KC/GRO, TNF-α, VEGF, EPO, GM-CSF, IFN-γ in both serum and BAL fluid were measured using a commercial U-PLEX Biomarker Group 1 Mouse 35-Plex (MesoScale Discovery) platform following the manufacturers' instructions; mouse IL-28 (IFN-k 2/3), mouse G-CSF, mouse TRAIL, mouse AREG were detected using ELISA kits (RayBiotech, Inc,); Oncostatin M and mouse surfactant protein D (SPD) were measured using Quantikine kits from R&D Systems following the manufacturer's instructions. .. Mouse IFN-α was measured in serum and BAL fluid by mouse IFN alpha platinum ELISA (ThermoFisher scientific) Serum and BAL fluid levels of mouse interferon-β (IFN-β) were measured using VeriKine Mouse Interferon Beta HS ELISA Kits (PBL Assay Science).

    Article Title: Mechanism of resistance to phagocytosis and pulmonary persistence in mucoid Pseudomonas aeruginosa
    Article Snippet: .. IL-8 or MIP-2 levels were determined by ELISA using Quantikine kits (R&D Systems d8000c, mm200) as described by the manufacturer. ..

    Article Title: Progesterone modulates TNF receptors expression by Jurkat cells cultured with plasma from pregnant women with preeclampsia.
    Article Snippet: The results were analyzed in FlowLogic Software Product (Inivai Technologies Pty Ltd, Australia). .. The concentration of sTNFR1, sTNFR2, and TNF in the plasma obtained from pregnant women with PE and from NT pregnant women, as well as sTNFR1, sTNFR2, TNF IL-10 in the supernatant of Jurkat cells, P.B. da Silva et al. International Immunopharmacology 130 (2024) 111701 treated with or without progesterone and plasma from pregnant women with PE or NT pregnant women was determined by enzyme-linked immunosorbent assay (ELISA) using Quantikine kits (R&D Systems). ..

    other:

    Article Title: Androgen signaling restricts glutaminolysis to drive sex-specific Th17 metabolism in allergic airway inflammation
    Article Snippet: Homogenized left lungs or BAL supernatants were used to measure IL-13 and IL-17A levels with Quantikine kits (R&D) per manufacturer’s instructions.

    Article Title: Antiphospholipid antibodies are associated with increased levels of selected oxidative stress biomarkers.
    Article Snippet: For quantification of both biomarkers, the Quantikine kits for human cytokines (i.e., IL-6 - Catalog #: D6050B, TNF-α - Catalog #: DTA00D, respectively; R&D Systems Inc., Minneapolis, MN, USA) were used.

    Article Title: Antiphospholipid antibodies are associated with increased levels of selected oxidative stress biomarkers
    Article Snippet: For quantification of both biomarkers, the Quantikine kits for human cytokines (i.e., IL-6 - Catalog #: D6050B, TNF-α - Catalog #: DTA00D, respectively; R&D Systems Inc., Minneapolis, MN, USA) were used.

    Bacteria:

    Article Title: Inhibition of Toxic Shock Syndrome-Associated Staphylococcus aureus by Probiotic Lactobacilli
    Article Snippet: .. Subsequently, the cells and bacteria were frozen at −20°C for 24 h. Next, IL-8 was measured using Quantikine kits from R&D Systems (Minneapolis, MN). ..

    Concentration Assay:

    Article Title: Progesterone modulates TNF receptors expression by Jurkat cells cultured with plasma from pregnant women with preeclampsia.
    Article Snippet: The results were analyzed in FlowLogic Software Product (Inivai Technologies Pty Ltd, Australia). .. The concentration of sTNFR1, sTNFR2, and TNF in the plasma obtained from pregnant women with PE and from NT pregnant women, as well as sTNFR1, sTNFR2, TNF IL-10 in the supernatant of Jurkat cells, P.B. da Silva et al. International Immunopharmacology 130 (2024) 111701 treated with or without progesterone and plasma from pregnant women with PE or NT pregnant women was determined by enzyme-linked immunosorbent assay (ELISA) using Quantikine kits (R&D Systems). ..

    Clinical Proteomics:

    Article Title: Progesterone modulates TNF receptors expression by Jurkat cells cultured with plasma from pregnant women with preeclampsia.
    Article Snippet: The results were analyzed in FlowLogic Software Product (Inivai Technologies Pty Ltd, Australia). .. The concentration of sTNFR1, sTNFR2, and TNF in the plasma obtained from pregnant women with PE and from NT pregnant women, as well as sTNFR1, sTNFR2, TNF IL-10 in the supernatant of Jurkat cells, P.B. da Silva et al. International Immunopharmacology 130 (2024) 111701 treated with or without progesterone and plasma from pregnant women with PE or NT pregnant women was determined by enzyme-linked immunosorbent assay (ELISA) using Quantikine kits (R&D Systems). ..

    Cytotoxicity Assay:

    Article Title: HER2-CD3-Fc Bispecific Antibody-Encoding mRNA Delivered by Lipid Nanoparticles Suppresses HER2-Positive Tumor Growth.
    Article Snippet: .. The medium collected from the real-time cytotoxicity assay was analyzed for human granzyme B, IFN-λ, and TNF-α secretion levels using Quantikine kits (R&D Systems, Minneapolis, MN, USA), according to the manufacturer’s protocols. ..



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    Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with <t>ELISA</t> across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) <t>and</t> <t>IL-8</t> (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.
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    Image Search Results


    T-cell migration and activity. A T-cell migration towards a FCS gradient or conditioned medium from B16BL6 melanoma cells. Pan T-cells were isolated from the spleens of wildtype (WT) and IKKε KO mice and plated into cell culture insert filled with T-cell medium containing 0.1% FCS. The bottom chamber was filled either with medium containing 0.1% FCS (neg ctrl), 10% FCS (pos ctrl) or conditioned medium from B16BL6 cells without FCS (B16). Cell migration of different T-cell subtypes to the lower channel was determined by FACS analysis. Cell numbers were normalized to counting beads and negative controls. n = 3–6. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01. B — D Effects of co-culture of B16BL6 melanoma cells with CD8⁺ and γδ-T-cells, respectively, derived from the spleens of wildtype (WT) and IKKε KO mice. B Cell proliferation determined by WST assay, B16BL6 monocultures were set as 1 for better comparison. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, **P < 0.01 significant difference in comparison to B16BL6 monoculture ( C ) IFN-γ levels analyzed by cytokine bead array (CBA) and ( D ) granzyme B release determined by ELISA. n = 4–10/group. C and D The T-cell monocultures of wildtype and IKKε knock-out mice were set as 1 for better comparison. Student´s t-test, * P < 0.05, significant difference in comparison to wildtype, # P < 0.05, ## P < 0.01, ### P < 0.001 significant difference in comparison to T-cell monocultures

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: T-cell migration and activity. A T-cell migration towards a FCS gradient or conditioned medium from B16BL6 melanoma cells. Pan T-cells were isolated from the spleens of wildtype (WT) and IKKε KO mice and plated into cell culture insert filled with T-cell medium containing 0.1% FCS. The bottom chamber was filled either with medium containing 0.1% FCS (neg ctrl), 10% FCS (pos ctrl) or conditioned medium from B16BL6 cells without FCS (B16). Cell migration of different T-cell subtypes to the lower channel was determined by FACS analysis. Cell numbers were normalized to counting beads and negative controls. n = 3–6. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01. B — D Effects of co-culture of B16BL6 melanoma cells with CD8⁺ and γδ-T-cells, respectively, derived from the spleens of wildtype (WT) and IKKε KO mice. B Cell proliferation determined by WST assay, B16BL6 monocultures were set as 1 for better comparison. One-way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, **P < 0.01 significant difference in comparison to B16BL6 monoculture ( C ) IFN-γ levels analyzed by cytokine bead array (CBA) and ( D ) granzyme B release determined by ELISA. n = 4–10/group. C and D The T-cell monocultures of wildtype and IKKε knock-out mice were set as 1 for better comparison. Student´s t-test, * P < 0.05, significant difference in comparison to wildtype, # P < 0.05, ## P < 0.01, ### P < 0.001 significant difference in comparison to T-cell monocultures

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Migration, Activity Assay, Isolation, Cell Culture, Analysis, Counting, Co-culture Assay, Derivative Assay, WST Assay, Comparison, Enzyme-linked Immunosorbent Assay, Knock-Out

    Regulation of cytokines and angiogenesis markers in the tumor. A OLINK proteomic analysis of inflammatory cytokines and chemokines in the tumors of wildtype and IKKε KO mice at the end of the experiment. Heat map and Volcano Plot of significantly different regulations. n = 4/group ( B ) Cxcl1 and Cxcl2 ELISA. Cxcl1: WT n = 8, IKKε-KO n = 6; Cxcl2: WT n = 6, IKKε-KO n = 5. C Determination of CD31-positive cells as vessel markers in the tumors. Left panel: FACS analysis, n = 6 per group. Right panel: immunofluorescence images and quantitative analysis, n = 4–5 tumors per group; 3–5 areas per tumor were evaluated. CD31-positive areas were normalized to wildtype controls to facilitate comparison. D MMP-9 expression as assessed by Western blot and zymography, n = 5–6 per group. E HIF-1α expression as determined by immunofluorescence, the pictures show on representative stain from 6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. F VEGF expression analyzed by ELISA and Western Blot, n = 3/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: Regulation of cytokines and angiogenesis markers in the tumor. A OLINK proteomic analysis of inflammatory cytokines and chemokines in the tumors of wildtype and IKKε KO mice at the end of the experiment. Heat map and Volcano Plot of significantly different regulations. n = 4/group ( B ) Cxcl1 and Cxcl2 ELISA. Cxcl1: WT n = 8, IKKε-KO n = 6; Cxcl2: WT n = 6, IKKε-KO n = 5. C Determination of CD31-positive cells as vessel markers in the tumors. Left panel: FACS analysis, n = 6 per group. Right panel: immunofluorescence images and quantitative analysis, n = 4–5 tumors per group; 3–5 areas per tumor were evaluated. CD31-positive areas were normalized to wildtype controls to facilitate comparison. D MMP-9 expression as assessed by Western blot and zymography, n = 5–6 per group. E HIF-1α expression as determined by immunofluorescence, the pictures show on representative stain from 6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. F VEGF expression analyzed by ELISA and Western Blot, n = 3/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Analysis, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Comparison, Expressing, Western Blot, Zymography, Staining

    Early regulation of cytokines and angiogenesis markers in the tumor. A Cxcl1 ELISA, ( B ) MMP-9 expression (Western Blot and zymography), ( C ) HIF-1α expression as determined by immunofluorescence. The pictures show a representative stain from 4–6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. D FACS analysis of CD31. + -cells as vessel markers in the tumors, ( n = 6/group). E VEGF expression analyzed by ELISA and Western Blot, ( F ) p65 expression and activity in nuclear extracts as determined by Western Blot and transcription factor ELISA. n = 3–5/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: Early regulation of cytokines and angiogenesis markers in the tumor. A Cxcl1 ELISA, ( B ) MMP-9 expression (Western Blot and zymography), ( C ) HIF-1α expression as determined by immunofluorescence. The pictures show a representative stain from 4–6 independent analyses, the diagram shows the densitometric analysis of all experiments, scale bar represents 100 µm. D FACS analysis of CD31. + -cells as vessel markers in the tumors, ( n = 6/group). E VEGF expression analyzed by ELISA and Western Blot, ( F ) p65 expression and activity in nuclear extracts as determined by Western Blot and transcription factor ELISA. n = 3–5/group. Student´s t-test, * P < 0.05, ** P < 0.01

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Enzyme-linked Immunosorbent Assay, Expressing, Western Blot, Zymography, Immunofluorescence, Staining, Analysis, Activity Assay

    Angiogenesis assay in HUVEC with and without depletion of IKKε by siRNA. A IKKε mRNA and protein expression in HUVEC cells after treatment with IKKε siRNA or a scrambled negative control as determined by PCR and Western Blot analysis, PCR: n = 3–4/group, Western Blot ( n = 2/group) ( B ) Tube formation assay, left side: microscopic pictures, right side: analysis of the total mesh area of naïve HUVEC, or HUVEC treated with NC and IKKε siRNA. n = 6–7/group. The scale bars represent 100 µm. C Spheroid sprout assay, left side: microscopic pictures, right side: analysis of cumulative sprout length (CSL) in spheroids of naïve HUVEC ( n = 15 spheroids), HUVEC treated with VEGF ( n = 38 spheroids) HUVEC-NC ( n = 25 spheroids) and HUVEC-IKKε siRNA ( n = 38 spheroids). Data were generated in 5 independent biological experiments. The scale bars represent 100 µm. One Way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Journal: Molecular Medicine

    Article Title: The protein kinase IKK epsilon shapes the melanoma tumor microenvironment by modulating angiogenesis and the T-cell immunity

    doi: 10.1186/s10020-026-01651-w

    Figure Lengend Snippet: Angiogenesis assay in HUVEC with and without depletion of IKKε by siRNA. A IKKε mRNA and protein expression in HUVEC cells after treatment with IKKε siRNA or a scrambled negative control as determined by PCR and Western Blot analysis, PCR: n = 3–4/group, Western Blot ( n = 2/group) ( B ) Tube formation assay, left side: microscopic pictures, right side: analysis of the total mesh area of naïve HUVEC, or HUVEC treated with NC and IKKε siRNA. n = 6–7/group. The scale bars represent 100 µm. C Spheroid sprout assay, left side: microscopic pictures, right side: analysis of cumulative sprout length (CSL) in spheroids of naïve HUVEC ( n = 15 spheroids), HUVEC treated with VEGF ( n = 38 spheroids) HUVEC-NC ( n = 25 spheroids) and HUVEC-IKKε siRNA ( n = 38 spheroids). Data were generated in 5 independent biological experiments. The scale bars represent 100 µm. One Way ANOVA with Tukey´s multiple comparisons test, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001

    Article Snippet: Granzyme B, Cxcl1, Cxcl2 and VEGF protein levels were determined from cell and tumor protein lysates using Mouse Granzyme B ELISA Kit (Invitrogen/Thermo Fisher Scientific, Dreieich, Germany #BMS6079), Mouse CXCL1/KC Quantikine ELISA Kit (#MKC00B), Mouse CXCL2/MIP-2 DuoSet ELISA (#DY452) and Mouse VEGF Quantikine ELISA Kit (#MMVOO-1) (all R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.

    Techniques: Angiogenesis Assay, Expressing, Negative Control, Western Blot, Analysis, Tube Formation Assay, Spheroids, Generated

    Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with ELISA across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) and IL-8 (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.

    Journal: Frontiers in Immunology

    Article Title: The role of NEDD4-mediated ubiquitination of AQP5 in allergic rhinitis: implications for nasal mucosal barrier function

    doi: 10.3389/fimmu.2026.1908261

    Figure Lengend Snippet: Disruption of the NEDD4-AQP5 axis impairs barrier function and promotes cytokine release. (A) MUC5AC amounts (pg/mL) in cellular culture supernatants were assayed with ELISA across various treatment groups. (B) TER (Ω·cm²) of HNEpC monolayers was measured utilizing a Millicell ERS-2 system across the specified groups. (C, D) The amounts of IL-6 (pg/mL) and IL-8 (pg/mL) in cellular culture supernatants were determined via ELISA. Data are shown as mean ± SD from three independent experiments, with each sample measured in triplicate. *P < 0.05, **P < 0.01, ***P < 0.001 vs. Ctrl group; # P < 0.05, ## P < 0.01, ### P < 0.001 compared with the HDM group. Ctrl, untreated control; HDM, house dust mite-sensitized HNEpCs; HDM + siNEDD4, HDM-sensitized HNEpCs transfected with siNEDD4; HDM + ovAQP5, HDM-sensitized HNEpCs transfected with AQP5 overexpression plasmid.

    Article Snippet: Cell culture supernatants were used to detect IL-6, IL-8, and MUC5AC levels in the supernatant by using ELISA kits for IL-6 (R&D Systems, D6050), IL-8 (R&D Systems, D8000C), and MUC5AC (R&D Systems, DY2197).

    Techniques: Disruption, Cell Culture, Enzyme-linked Immunosorbent Assay, Control, Transfection, Over Expression, Plasmid Preparation